| Home | E-Submission | Sitemap | Contact Us |  
Environ Anal Health Toxicol > Volume 36:2021 > Article
Jang, Park, Im, Park, Seo, Park, and Nah: In vitro acute inhalation toxicity for TiO2 (GST) using 3D human tissue model (EpiAirway™)


The present study was performed to screen in vitro potential acute inhalation toxicity using an EpiAirway tissue model (human tracheal/bronchial tissue) for the nano-sized titanium dioxide, GST manufactured as a photocatalyst through of sludge recycling and to compare with P-25 a commercialized photocatalytic material. According to the protocol provided by in vitro tissue manufacturer, the GST was exposure to the tissue for 3 hours in 450, 500, 650, 850 mg/mL concentration after preliminary dose range finding study and then tissue viability (%, IC75) was calculated using the MTT assay. Besides, the histopathological observation was performed to compare to the MTT assay. As a result of study, IC75 could not be confirmed at 850 mg/mL in both GST and P-25 and the grade was confirmed to be IC75> 600 mg/mL in vitro model tissue category. Therefore, it was considered that the GHS category could be classified as ‘No classification’ in screening method for potential acute inhalation toxicity. Also, not the morphological effects of epithelial cells in tissue model were observed compared with the vehicle control and histological findings were similar to the results of MTT Viability assay. Based on these results, the potential acute inhalation toxicity for GST produced through sludge recycling using in vitro tissue model inhalation toxicity showed that it could be non-hazardous substance. However, further study (in vivo study, etc.) is thought to be needed to ascertain whether GST is a toxic effect or safe.


The TiO2 nanoparticles (NPs) has been widely used in a variety of products including consumer products, paints, pigments, orthodontic composites, food and cosmetic products [14]. In addition, TiO2 NPs are produced abundantly and used widely because of high stability, anticorrosive and photocatalytic properties [56].
For this reason, the toxicological studies of TiO2 NPs have been currently performed with several routes including dermal, oral and inhalation [714]. Especially, it is important to assess the risk of respiratory tract serves as both target tissue and portal of entry to the systemic circulation for inhaled substances. In inhalation toxicity study, most of the studies have been reported with the P25 from, which consists of TiO2 NPs (anatase structure: 80~90%, rutile structure: 20~10%), generally used in catalytic and photocatalytic industrial application [7].
In test guidelines, the study of acute inhalation toxicity potential is important for establishing safe handing, package, labeling, transport, and use procedures for chemicals. The commonly accepted test of acute inhalation for GHS (Globally Harmonized System) category has been in vivo animal study; OECD test guidelines 403 (acute inhalation toxicity test), 433 (acute inhalation toxicity: Fixed concentration procedure), 436 (acute inhalation toxicity: acute toxic class method) [1517]. However, the limitations in animal study have been demonstrated for interspecies differences in the respiratory tract architecture and function and it is difficult that the conclusions are drawn on the potential hazard of inhaled compounds in human [18]. Recently, a new test for determining acute inhalation toxicity has been brought forward for validation and OECD adoption. This test adopts the EpiAirway model, a ready-to-use, three-dimensional (3D) in vitro muco-ciliary tissue model consisting of normal, human-derived tracheal/bronchial epithelial cells cultured at the Air-Liquid Interface (ALI) and pre-validation results for 59 chemicals showed that it was a promising human-relevant in vitro alternative to animal tests for acute inhalation toxicity studies. This pre-validation data indicated that GHS acute inhalation toxicity category 1–2 had IC75 toxicity values < 150 mg/mL, category 3 had IC75 toxicity values 150 mg/mL < IC75≤400 mg/mL, category 4–5 had IC75 toxicity values 400 mg/mL < IC75≤ 600 mg/mL based on combined GHS acute inhalation plus STOT (Specific Target Organ Toxicity) [19]. Chemicals that have been evaluated and determined to be nonhazardous has IC75 toxicity values> 600 mg/mL.
Recently, the study was conducted to produce TiO2 from the wastewater sludge generated by the flocculation of secondary wastewater with titanium tetrachloride (TiCl4) [20]. Also, Gong JH et al. conducted a study that TiO2 photocatalyst was produced from the precipitated sludge using TiCl4 coagulants in the sewage treatment plants (Changwon-si, Gyeongsangnam-do, Republic of Korea) and it had cost-competitive lower than price of commercial photocatalyst (P-25) [21].
The aim of the present study was conducted using in vitro human 3D model (EpiAirway) as a screening method for the potential acute inhalation toxicity for TiO2 photocatalyst (GST) produced through sludge recycling of the sewage treatment plants. Also, we made a comparison with P-25 form, the most commonly tested commercial substance and the histopathological examinations were performed for model tissue.

Materials and Methods

Test substance

The GST (pale yellow powder) was manufactured from the precipitated sludge using TiCl4 coagulants in the sewage treatment plants and P-25 (commercial nano-particle products, white powder; Evonic, Germany) was provided by Bentech Frontier Co. Ltd. The crystalline composition in X-ray diffraction (XRD) analysis was performed by the Research Institute for Catalysis in Chonnam National University and the crystalline ratio was 100% anatase and 88% anatase / 12% rutile in GST, P-25, respectively.
The measurements of zeta potential, particle size image (SEM, scanning electron microscope) and TEM (Transmission Electron Microscopy) image, size distribution for GST were performed as follows and were presented in (Figure 1 and Figure 2), respectively. In zeta potential, it indicated negative zeta potential value (−35.4 mV), which is an indirect parameter for surface charge. A more negative zeta potential favors the particle agglomeration less rapidly. This value showed that the charge of the hydrated particles was not high enough to repel each other by electrostatic forces. Also, the measured particle sizes including size distribution indicated that the particles of GST had a distribution of various size (95.8±46.3 nm, >100 nm-34.1%).
  • For zeta potential for surface analysis – particle size & zeta potential analyzer (Zetasizer Nano ZSP, Malvern Instruments LTD., UK) in Korea TECH

  • For particle size analysis - FE-SEM(Field Emission Scanning Electrong Microscope, Tescan Corp., Czech) equipped with EDS systems (Thermo scientific, USA) in KRICT (Ulsan department)

  • For TEM image – FE-EF-TEM (Field Emission Energy Filtered Transmission Electron Microscopy, JEOL, Japan) in Korea Basic Science Institute (Jeonju department)

  • For size distribution analyze of TEM image – ImageJ software (https://imagej.nih.gov/ij/download.html)

In addition, the acetonitrile (Burdick and Jackson., Cas No. 75-05-8), one of 59 chemicals used for pre-validation study was selected to verify reproducibility of IC75 before performing the study of TiO2 NPs (GST / P-25, Cas No. 134363-67-7) [19]. In the confirmation test for the vehicle, GST and acetonitrile were identified as sterile distilled water (D.W.), and P-25 was 20% acetone (SIGMA-Aldrich, Lot No.: MKCK2599)/olive-oil (SIGMA-Aldrich, Lot No.: BCBT7822) (1:4 v/v%). The TiO2 NPs were suspended in vehicles and it was used after stirring and additional sonication to disperse. As a positive control, formaldehyde solution (SIGMA-Aldrich, Lot No.: 47033-U, ≥36.0% in H2O) was used for TiO2 NPs (GST, P-25).
The sodium dodecyl sulfate solution (SDS) was selected considering to be a substance known as irritant for inhalation, skin, eye [22] and 10% SDS (SIGMA-Aldrich, Lot No.: BCCB7687) was diluted to 5% (v/v) in PBS (Phosphate Buffered Saline, Lonza Ltd., Lot No. 0000725114) and then used as positive control of acetonitrile [23]. Vehicles were used as the negative control group (D.W., acetone/olive-oil (1:4)).

3D In vitro respiratory tissue model (EpiAirway)

Preparation of tissue

The EpiAirway tissue model was produced by MatTek Corporation (Ashland, MA, USA) and purchased through Dail Scientific Trade, Inc. in Korea. The components included tissue (AIR-100), assay kit (assay medium; AIR-100-ASY, AIR-100-MM, buffer; TEER Buffer), and MTT kit (diluent; MTT-100-DIL, concentrate; MTT-100-CON, extractant; MTT-100-EXT). After receiving the tissue, the insert membrane was separated from agarose and was transferred in to 6 well plate. Then 1.0 mL of cold medium was dispersed into each well of the 6-well plates provided and the tissue was equilibrated overnight (37°C, 5% CO2 16–18 hours).

Preliminary dose range finding (DRF) test

The test was carried out in accordance with the EpiAirway toxicity testing protocol. 100 uL of test substance were applied to the apical surface of each tissue; 0 (vehicle only), 10, 50, 250 and 500 mg/mL. The tissues with test substance were incubated for 3 hours (37°C, 5% CO2 incubation, n=3 tissue/dose) and then the MTT viability for IC75 value was performed. In the case of acetonitrile, the DRF test was omitted, referring to the pre-validation data (IC75; 284.72±72.1 mg/mL) [19].

Definitive toxicity test

Aa a result of the DRF test, the viability for TiO2 NPs, GST and P-25 was IC75>500 mg/mL and definitive doses were determined in accordance with the EpiAirway toxicity testing protocol; 0 (vehicle only), 450, 650, 850 mg/mL (Table 1). In addition, one dose (the high dose of the DRF, 500 mg/mL) was included to check the reproducibility.
The formaldehyde was determined as a positive control according to the EpiAirway toxicity testing protocol. In Acetonitrile, the determined doses were 0 (negative control only), 200, 300, 420, and 550 mg/mL considering IC75 data of pre-validation. The detailed dose determination is explained in (Table 1). The test substances were treated in the same way as preliminary dose range finding test; incubation for 3 hours (37°C, 5% CO2 incubation, n=3 tissue/dose) and MTT assay for viability.

MTT viability assay

EpiAirway tissues (Air-100) were transferred to 24-well culture plates containing 300 uL of MTT reagent per well and incubated in a 37C, 5% CO2 incubator for 3 hours. After incubation, the tissues were submerged in 2.0 mL of MTT extractant and extracted into shaker for 2 hours. For extraction time the plate was sealed to protect against light and prevent evaporation of MTT extractant. And then 200 uL of extractant from each tissue was transferred to a clear 96-well plate and absorbance was measured at 570 nm with background at 650 nm subtracted.
The viability of test substance-exposed tissues was calculated relative to negative controls using the equation: Relative viability (%) = [OD test substance / Mean OD negative control]×100. All control and treatment groups included n=3 replicates. Also, GraphPad Prism software was used to calculate the inhibitory concentration values (IC75; the dose required to reproduce the EpiAirway culture viability to 75% of vehicle-treated tissues) were calculated using Prism software (USA).


Histopathological examination was performed to observe morphological changes in tissues according to cell viability. For this, one tissue from each treatment group, including the negative control tissues, was fixed in 10% neutral buffered formalin (BBC Biochemical, USA, Lot No.95922) (overnight, room temperature) and the tissues were embedded with paraffin. The tissue sections were adhered to slides and were stained by hematoxylin (BBC Biochemical, USA, Lot No.95925) and eosin (BBC Biochemical, USA, Lot No.959058) (H&E) for observation under light microscopy.

Results and Discussion

Preliminary dose range finding (DRF) test

The average MTT viability of GST was 88.5%, 94.1%, 97.3%, 88.8% and P-25 was 104.6%, 98.4%, 110.9%, 104.6% in concentration of 10, 50, 250, 500 mg/mL, respectively. As a result of viability, TiO2 NPs (GST and P-25) had a MTT viability of 75% higher. Also, the optical density values were 2.0 or higher [negative control optical density should be ≥ 1.455: value – 2.579 (GST), 2.084 (P-25)]. These results indicate that there was no difference between the GST and P-25. IC75 could not be calculated that cell viability was observed more than 75% in all doses. The detailed evaluation of relative viability and optical values for the preliminary dose range finding test is depicted and detailed in (Figure 1, Figure 2 and Table 2).

Definitive toxicity test

The average MTT viability of GST was 92.1%, 93.3%, 99.8%, 96.4% and P-25 was 96.7%, 87.3%, 102.1%, 86.8% in concentration of 450, 500, 650, 850 mg/mL and the positive control (formaldehyde) was 0.7%, 1.6%, respectively. The results of the MTT Viability assay for GST and P-25 meant that IC75 values could not be calculated at 850 mg/mL (high dose) and the grade was IC75 > 600 mg/mL in EpiAirway in vitro model considering as follows.
Although EpiAirway in vitro model has not yet been adopted as OECD test guideline for acute inhalation toxicity, it was considered that the study using IC75 value could be a screening method for GHS categorization. Therefore, it was expected that the category for GST would not be classified, and no potential toxicity was considered.
In the case of P-25, commercial product, the safety data sheet (SDS) of manufacturer (Evonik Corp.) showed that LC50 (rat) was > 6.82 mg/L in dusts, mists and fumes vapour route. It indicates that GHS classification was considered to be category 3 (2.0 < acute toxicity estimate≤ 10.0, vapour) or category 5 (dusts, mists). Based on the these, GST substance was considered to be similar with P-25 in inhalation route and further study (in vivo study, etc.) is thought to be needed to ascertain whether GST shows a toxic effect or safe.
The inhaled nanoparticles are mainly found in the upper airways (nose, mouth, pharynx, larynx and trachea) but it can reach the deeper lungs and deposit in alveoli [24]. In the study for assessment pulmonary effects, three studies for rodents reported irritation and mild or moderate pulmonary inflammation [1214]. Also, the study using mice (8.88 mg/m3 for 4h/day) showed higher counts of total cells and alveolar macrophage in the BALF (bronchoalveolar lavage fluid) [25] and the study exposed to aerosols in rats (2, 10, 50 mg/m3 for 6hr/day) showed a lung inflammation with dose-dependent increases in BALF [26]. In this study, we performed this study to screen the potential acute inhalation toxicity for GST using the EpiAirway model (tracheal/bronchial tissue) and this result indicates that TiO2 NPs, GST produced through sludge recycling is considered to be safe comparing with the reference studies.
In acetonitrile test, the average MTT viability was 65.2%, 19.3%, 7.5%, 2.8% in concentration of 200, 300, 420, 550 mg/mL and IC75 was calculated as 183.6 mg/mL. This result showed that grade was 150 < IC75 ≤400 mg/mL in 3D in vitro model and GHS was considered to be category 3. Though GHS acute classification was known for category 4 (in vivo, in male CD-1 mice, LC50 2,693 ppm), it was identified in the same category 3 in 3D in vitro model as the pre-validation data (IC75: 284.72±72.1 mg/mL [19, 27]. The difference in a 3D model based on GHS-STOT classification and in vivo data for GHS category was considered to be due to the difference in species; GST-STOT classification based on the human data and tissue with human-derived tracheal/bronchial epithelial cells in EpiAirway model, animal data (mouse) in vivo study.
The detailed evaluation of relative viability and optical values for the Definitive toxicity test of relative viability is depicted and detailed in (Figure 3 and Table 3). The positive control (SDS) was 3.8% cell viability and it showed the strong irritation for tissue model.


Histopathological observations showed that the epithelial cell layer effects for TiO2 NPs (GST and P-25) were not observed in tissue model and it was similar with negative control (D.W). However, the cell layer was almost entirely damaged and eliminated in positive control (formaldehyde solution). These changes in the histopathological examination were observed to be similar to results of MTT tissue viability (%). The histopathological findings were presented in (Figure 4 and Figure 5).
In tissue observation for acetonitrile, the damages for cell layer were confirmed in 200 mg/mL and 550 mg/mL, respectively as (Figure 6) (C, D). These effects showed dose-dependent tendency and was similar to results of MTT tissue viability (%); destruction of cell layer in 200 mg/mL concentration, elimination of cell layer in 550 mg/mL (Figure 7).


When the TiO2 NPs (GST) was tested with an EpiAirway tissue model (human tracheal/bronchial tissue) as a screening method for in vitro acute inhalation toxicity, it was concluded that IC75 could not be confirmed up to 850 mg/mL in MTT viability and no histopathological effect was observed for tissue model. Also, EpiAirway model application appears to be a promising approach for in vitro determination of acute inhalation toxicity testing to facilitate moderately high-throughput screening. These results for GST were similar with that of P-25, commercial product under this condition. Therefore, on the basis of results of in vitro tissue model for GST manufactured as a photocatalyst through of sludge recycling, it was considered that GST could be classified as non-hazardous substance in inhalation route.


This work was supported by a grant (19SCIP-B145906-02) from the Korea Agency for Infrastructure Technology Advancement (KAIA) by Ministry of Land, Infrastructure and Transport of Korea government (MOLIT), Republic of Korea.

Conflict of interest

The authors declare that they have no conflict of interest.


CRediT author statement
SYJ: Methodology, Data curation, Writing-Original draft preparation; MKP: Supervision, Writing-Reviewing and Editing; JMI: Visualization; HSP: Visualization; HSS: Visualization; HJP: Resources; SSN: Project administration, Writing-Reviewing and Editing.


1. Taavitsainen VM, Jalava JP. Soft and harder multivariate modeling in developing the properties of titanium dioxide pigments. Chemometr Intell Lab Syst 1995;29(2):307-319 https://doi.org/10.1016/0169-7439(95)80105-I.
2. Sun D, Meng TT, Loong TH, Hwa TJ. Removal of natural organic matter from water using a nano-structured photocatalyst coupled with filtrating membrane. Water Sci Technol 2004;49(1):103-110 https://doi.org/10.2166/wst.2004.0030.
3. Marta B, Gabriela K, Marcin O, Wieslaw L, Wojciech M. Singlet oxygen generation in the presence of titanium dioxide materials used as sunscreens in suntan lotions. Journal of Photochemistry and Photobiology A: Chemistry 2010;213(2–3):158-163 https://doi.org/10.1016/j.jphotochem.2010.05.019.
4. Winkler HC, Notter T, Meyer U, Naegeli H. Critical review of the safety assessment of titanium dioxide additives in food. J Nanobiotechnol 2018;16(51):https://doi.org/10.1186/s12951-018-0376-8.
5. Shi H, Magaye R, Castranova V, Zhao J. Titanium dioxide nanoparticles: a review of current toxicological data. Part Fibre Toxicol 2013;10(1):1-33 https://doi.org/10.1186/1743-8977-10-15.
crossref pmid pmc
6. Oberdorster G. Pulmonary effects of inhaled ultrafine particle. Int Arch Occup Environ Health 2000;74(1):1-8 https://doi.org/10.1007/s004200000185.
7. Dreno B, Alexis A, Chuberre B, Marinovich M. safety of titanium dioxide nanoparticles in cosmetics. J Eur Acad Dermatol Venereol 2019;33: 34-46 https://doi.org/10.1111/jdv.15943.
8. Auttachoat W, McLoughlin CE, White KL Jr, Smith MJ. Route-dependent systemic and local immune effects following exposure to solutions prepared from titanium dioxide nanoparticles. J Immunotoxicol 2014;11(3):273-282 https://doi.org/10.3109/1547691X.2013.844750.
crossref pmid
9. Warheit DB, Brown SC, Donner EM. Acute and subchronic oral studies in rats with nanoscale and pigment grade titanium dioxide particles. Food Chem Toxicol 2015;84: 208-224 https://doi.org/10.1016/j.fct.2015.08.026.
crossref pmid
10. Hu R, Gong X, Duan Y, Li N, Che Y, Cui Y, et al. Neurotoxicological effects and the impairment of spatial recognition memory in mice caused by exposure to TiO2 nanoparticles. Biomaterials 2010;31(31):8043-8050 https://doi.org/10.1016/j.biomaterials.2010.07.011.
crossref pmid
11. Sang X, Li B, Ze Y, Hong J, Ze X, Gui S, et al. Toxicological mechanisms of nanosized titanium dioxide-induced spleen injury in mice after repeated peroral application. J Agric Food Chem 2013;61(23):5590-5599 https://doi.org/10.1021/jf3035989.
crossref pmid
12. Leppanen M, Korpi A, Mikkonen S, Yli-Pirilä P, Lehto M, Pylkkänen L, et al. Inhaled silica-coated TiO2 nanoparticles induced airway irritation, airflow limitation and inflammation in mice. Nanotoxicology 2015;9(2):210-218 https://doi.org/10.3109/17435390.2014.914260.
crossref pmid
13. Noël A, Maghni K, Cloutier Y, Dion C, Wikinson KJ, Halle S, et al. Effects of inhaled nano-TiO2 aerosols showing two distinct agglomeration states on rat lungs. Toxicol Lett 2012;214(2):109-119 https://doi.org/10.1016/j.toxlet.2012.08.019.
crossref pmid
14. Oyabu T, Morimoto Y, Izumi H, Yoshiura Y, Tomonaga T, Lee BW, et al. Comparison between whole-body inhalation and nose-only inhalation on the deposition and health effects of nanoparticles. Environ Health Prev Med 2016;21(1):42-48 https://doi.org/10.1007/s12199-015-0493-z.
crossref pmid
15. OECD test guideline no. 403 acute inhalation toxicity; https://doi.org/10.1787/9789264070608-en.

16. OECD test guideline no. 433 acute inhalation toxicity: Fixed concentration procedure; https://doi.org/10.1787/9789264284166-en.

17. OECD test guideline no. 436 acute inhalation toxicity: Acute toxic class method; https://doi.org/10.1787/9789264076037-en.

18. Movia D, Bruni-Favier S, Prina-Mello A. In vitro alternatives to acute inhalation toxicity studies in animal models-a perspective. Front Bioeng Biotechnol 2020;8: 549 https://doi.org/10.3389/fbioe.2020.00549.
crossref pmid pmc
19. Jackson GR Jr, Maione AG, Klausner M, Hayden PJ. Prevalidation of an acute inhalation toxicity test using the EpiAirway in vitro human airway model. Appl In Vitro Toxicol 2018;4(2):149-158 https://doi.org/10.1089/aivt.2018.0004.
20. Shon HK, Vigneswaran S, Kim IS, Cho J, Kim GJ, Kim JB, et al. Preparation of titanium dioxide (TiO2) from sludge produced by titanium tetrachloride (TiCl4) flocculation of wastewater. Environ Sci Technol 2007;41(4):1372-1377 https://doi.org/10.1021/es062062g.
crossref pmid
21. Gong JH, Joo JC, Kim JK. Preparation and characteristic evaluation of Low-cost TiO2 photocatalyst. J Korean Soc Environ Eng 2019;41(4):196-203 https://doi.org/10.4491/KSEE.2019.41.4.196.
23. Fusco L, Garrido M, Martin C, Sosa S, Ponti C, Centeno A, et al. Skin irritation potential of graphene-based materials using a non-animal test. Nanoscale 2020;12(2):610-622 https://doi.org/10.1039/C9NR06815E.
crossref pmid
24. Kreyling WG, Scheuch G. Clearance of particles deposited in the lungs. In: Heyder J, Gehr P, editors. Particle Lung Interactions. Marcel Dekker; New York: 2000. p. 323-376.

25. Grassian VH, O’Shaughnessy PT, Adamcakova-Dodd A, Pettibone JM, Thorne PS. Inhalation exposure study of titanium dioxide nanoparticles with a primary particle size of 2 to 5 nm. Environ Health Perspect 2007;115(3):397-402 https://doi.org/10.1289/ehp.9469.
crossref pmid
26. Ma-Hock L, Burkhardt S, Strauss V, Gamer AO, Wiench K, van Ravenzwaay B, et al. Development of a short-term inhalation test in the rat using nano-titanium dioxide as a model substance. Inhal Toxicol 2009;21(2):102-118 https://doi.org/10.1080/08958370802361057.
crossref pmid
27. Willhite CC. Inhalation toxicology of acute exposure to aliphatic nitriles. Clin Toxicol 1981;18(8):991-1003 https://doi.org/10.3109/15563658108990329.
crossref pmid

Figure 1
Characterization of TiO2 particles (GST): (a) Zeta potential (−35.4 mV, 30 mg/mL); (b) SEM (scanning electron microscope) image.
Figure 2
Characterization of TiO2 particles (GST): (a) A particles dispersed in 99.9% EtOH was deposited on a copper grid and analyzed by TEM (Transmission electron microscope) image; (b) Size distribution of the imaged GST. The diameter measured was 95.8±46.3 nm (mean±standard deviation) by ImageJ and 34.1% by number of the particles had a diameter>100 nm.
Figure 3
Preliminary dose range finding test of relative viability; (a) GST of relative viability (b) P-25 of relative viability.
Figure 4
Definitive toxicity test of relative viability: (a) GST of relative viability; (b) P-25 of relative viability; (c) acetonitrile of relative viability.
Figure 5
EpiAirway tissue exposed to GST (H&E, HPF 400×): (A) Negative control (D.W); (B) Positive control (Formaldehyde solution); (C) 400 mg/mL concentration; (D) 850 mg/mL concentration.
Figure 6
EpiAirway tissue exposed to P-25 (H&E, HPF 400×): (A) Negative control (20% Acetone, acetone/olive-oil (1:4)); (B) Positive control (Formaldehyde solution); (C) 400 mg/mL concentration; (D) 850 mg/mL concentration.
Figure 7
EpiAirway tissue exposed to Acetonitrile (H&E, HPF 400×): (A) Negative control (D.W); (B) Positive control (5% sodium dodecyl sulfate); (C) 200 mg/mL concentration; (D) 550 mg/mL concentration.
Table 1
Doses for definitive determination based on results of DRF test [19].
DRF test result Dose of definitive toxicity test
IC75<10 mg/mL 0.1, 0.5 2.5 and 12.5 mg/mL
IC75>10< 50 mg/mL 5, 15, 30 and 60 mg/mL
IC75>50<250 mg/mL 40, 120, 200 and 280 mg/mL
IC75>250<500 mg/mL 200, 300, 420 and 550 mg/mL
IC75>500 mg/mL 450, 650, 850 mg/mL
Table 2
Evaluation of cell viability.
Group GST P-25

Dose (mg/mL) Optical Density Cell viability (%) Dose (mg/mL) Optical Density Cell viability (%)
G1 (Negative control) *-1 2.579 100.0 *-1 2.084 100.0
G2 (Test substance) 10 2.370 91.9 10 2.180 104.6
G3 (Test substance) 50 2.431 94.3 50 2.052 98.4
G4 (Test substance) 250 2.510 97.3 250 2.311 110.9
G5 (Test substance) 500 2.306 89.4 500 2.180 104.6

*-1 not applicable.

Table 3
Correlation of IC75 toxicity values with GHS Acute Inhalation Toxicity classification [19].
IC75 Toxicity values GHS Acute inhalation plus *1 STOT Classifications
IC75 ≤ 150 mg/mL GHS 1 – 2 Category
150 < IC75 ≤ 400 mg/mL GHS 3 Category
400 < IC75 ≤ 600 mg/mL GHS 4 – 5 Category
IC75 > 600 mg/mL No classification

*1 STOT: specific target organ toxicity

Table 4
Evaluation of cell viability.
GST P-25 Acetonitrile

Dose (mg/mL) Optical Density Cell Viability(%) Dose (mg/mL) Optical Density Cell Viability(%) Dose (mg/mL) Optical Density Cell Viability(%)
- 2.348 100.00 - 2.023 100.0 - 1.873 100.0
450 2.162 92.1 450 1.955 96.7 200 1.223 65.2
500 2.189 93.3 500 1.765 87.3 300 0.362 19.3
650 2.343 99.8 650 2.064 102.1 420 0.142 7.5
850 2.264 96.4 850 1.757 86.8 550 0.054 2.8
Positive control 1 0.017 0.7 Positive control 1 0.033 1.6 Positive control 2 0.073 3.8

1 Formaldehyde solution,

2 5% sodium dodecyl sulfate (SDS), - not applicable.

PDF Links  PDF Links
PubReader  PubReader
ePub Link  ePub Link
XML Download  XML Download
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Editorial Office
Division of Environmental Science and Ecological Engineering, Korea University
145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea
Tel : +82-32-560-7520   E-mail: envitoxic@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2024 by The Korean Society of Environmental Health and Toxicology & Korea Society for Environmental Analysis.     Developed in M2PI