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Journal of Environmental Toxicology 2003;18(4):295-303.
카드뮴에 대한 녹조류 Selenastrum capricomutum(Chlorophyceae)의 세포활력도 및 peroxidase 활성도 변화
최은주, 이상구, 이승진, 문성경, 박용석, 이기태
Changes in Cellular Viability and Peroxidase Activities of Green Algae Selenastrum capricornutum(Chlorophyceae) to Cadmium
Eun-Joo Choi , Sang-Goo Lee , Seung-Jin Lee , Seung-Kyung Moon , Yong-seok Park , Ki-tae Rhie
ABSTRACT
Physiological cellular activities responses to cadmium (Cd) exposure in green algae with several reductases activities and viability of the cell were examined. The cell division of green algae, Selenastrum capriconutum treated with 5ppm was significantly decreased than that of normal algae. The mean cell number of normal algal culture was as twice much as than that of algae at 6 days after Cd treatment. The cellular viability of algae was analysed by f1ow-cytometry with f1uorescent dye after esterase reaction on cell membrane. The 85.35% of cellular viability of normal culture was decreased to 34.35% when algae was treated with 5 ppm of Cd at 6 days after treatment. It was considered that those method of flow-cytometry is useful tool for toxicity test on micro-organisms in the respect of identifying cellular viability. Also, the activities of both glutathione peroxidase (GPX) and ascorbate peroxidase(APX), which are indirectly react against oxidative stress through reduction of glutathione by Cd were significantly increased with 25%. It is considered that both GPX and APX are involved in the metabolic pathway of Cd-detoxification with similar portion in Selenastrum capricornutum.
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