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Environ Anal Health Toxicol > Volume 37:2022 > Article
Environmental Analysis Health and Toxicology 2022;37(2):e2022011-0. doi: https://doi.org/10.5620/eaht.2022011
Protective action of polysaccharides from Laurencia papillose (Rhodophyta) against imidacloprid induced genotoxicity and oxidative stress in male albino rats
Hossam El Din H. Abdelhafez1 , Amr A AbdAllah1, Mostafa M Afify2 , Naglaa F Mahmoud3 , Jiangfeng Guo4 , Soha A Murad5 , Eman A Ibrahim6
1Mammalian and Aquatic Toxicology Department, Central Agricultural pesticides Lab, Agricultural Research Center,Giza,Egypt
2Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
3Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Cairo University, Giza, Egypt
4College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China
5Plant Biochemistry Department, National Research Centre (NRC), Giza, Egypt
6Plant Biochemistry Department, National Research Centre (NRC) , Giza, Egypt
Corresponding Author: Hossam El Din H. Abdelhafez ,Email: hossam4003@live.com
Received: November 1, 2021;  Accepted: April 19, 2022.
ABSTRACT
Imidacloprid (IMI), the main component of neonicotinoid insecticides, promotes oxidative stress and genotoxicity in mammals. The aim of this experiment is to assess oxidative stress in liver cells and genotoxicity of erythrocytes for rats exposed to sub-lethal doses of IMI and the protective effects for Rhodophyta as antioxidant material versus imidacloprid. A total of 30 adult male albino rats (average body weight, 190-200 g) were divided into six groups (n=5) as follows: group 1 served as the control, group 2 received 200 mg/kg red algae, group 3 received 45 mg/kg IMI (high-dose group), group 4 received 22.5 mg/kg IMI (low-dose group), group 5 received 200 mg/kg red algae +45 mg/kg IMI, and group 6 received 200 mg/kg red algae +22.5 mg/kg IMI. After 28 d of treatment, the antioxidant activity of the crude extract of red algae was assessed in terms of free radical scavenging activity and found to be higher in TCA (75.57%) followed by DPPH (50.08%) at concentration 100 μg extract and a significant increase in lipid peroxidation and reductions in glutathione were observed in liver cells were intoxicated with high and low doses of IMI. Moreover decreases in catalase and glutathione peroxidase parameters in same previous groups which indicated oxidative stress. In addition significant increases in micronucleus frequency (MN) in the bone marrow of the rats as a genotoxicity marker which indicated DNA damage in erythrocytes cells with alterations in the histopathology of liver cells were also noted such as necrosis, inflammatory cells, infiltration, and necrobiotic changes. Whereas Rhodophyta succeeded in alleviation the oxidative damage and genotoxicity induced by the insecticide. In conclusion, IMI demonstrates hazardous effects, such as alterations in antioxidant status and mutagenicity of erythrocytes and polysaccharides from Rhodophyta has good antioxidant activity in vivo model systems against imidacloprid.
Keywords: imidacloprid, oxidative stress, genotoxicity, micronucleus test, lipid peroxidation, hepatotoxicity
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